Difference between revisions of "Part:BBa K116201"

Revision as of 15:08, 29 September 2021

ureD promoter from P mirabilis

An 328 bp promoter region, which can be assembled with urea binding proteins to activate the downstream gene expression.

pUreD is an inducible promoter that is activated by the UreR transcription factor in the presence of urea. pUreD is one of several promoters activated by the UreR transcriptional activator which share a consensus sequence of TA/GT/CA/TT/GC/TTA/TT/AATTG [3].

pUreD is a bidirectional promoter driving transcription of the UreR and UreD protein subunits of the urease operon of P. mirabilis. pUreD contains two ribosome binding sites and a UreR binding site about upstream of the forward transcriptional start site.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]

12
COMPATIBLE WITH RFC[12]

21
COMPATIBLE WITH RFC[21]

23
COMPATIBLE WITH RFC[23]

25
COMPATIBLE WITH RFC[25]

1000
COMPATIBLE WITH RFC[1000]

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 An 328 bp promoter region, which can be assembled with urea binding proteins to activate the downstream gene expression.
+pUreD is an inducible promoter that is activated by the UreR transcription factor in the presence of urea. pUreD is one of several promoters activated by the UreR transcriptional activator which share a consensus sequence of TA/GT/CA/TT/GC/TTA/TT/AATTG [3].
+pUreD is a bidirectional promoter driving transcription of the UreR and UreD protein subunits of the urease operon of P. mirabilis. pUreD contains two ribosome binding sites and a UreR binding site about upstream of the forward transcriptional start site.
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 <partinfo>BBa_K116201 parameters</partinfo>
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-pUreD is an inducible promoter that is activated by the UreR transcription factor in the presence of urea. pUreD is one of several promoters activated by the UreR transcriptional activator which share a consensus sequence of TA/GT/CA/TT/GC/TTA/TT/AATTG [3].
-pUreD is a bidirectional promoter driving transcription of the UreR and UreD protein subunits of the urease operon of P. mirabilis. pUreD contains two ribosome binding sites and a UreR binding site about upstream of the forward transcriptional start site.