Difference between revisions of "Part:BBa K3804011"

Latest revision as of 08:48, 29 September 2021

Reporter for quorum sensing systems in yeast

We created this part with reference to BBa_K1024002in order to signal when Pseudomonas aeruginosa exists. The part is composed of pTEF, Flag, VP16, NLS, LasR, ADH1 terminator, pLasR, Cyc100mini, mCherry and CYC1terminator. We improved BBa_K1024002 by adding ADH1 terminator(BBa_J63002) and CYC1terminator(BBa_K1462070). The LuxR gene is constitutively expressed. On the other hand, activation of pLux occurs only when sufficient amounts of AHL exist in the environment. When pLux was activated, mCherry is expressed and lets us know the existence of AHL.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1580
Illegal BamHI site found at 2771
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 506
Illegal BsaI site found at 893
Illegal BsaI site found at 1280
Illegal BsaI.rc site found at 547
Illegal BsaI.rc site found at 934
Illegal BsaI.rc site found at 1321

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3804011 short</partinfo>
-We created this part with reference to BBa_K1024002in order to signal when Pseudomonas aeruginosa exists. The part is composed of pTEF, Flag, VP16, NLS, LasR, ADH1 terminator, pLasR, Cyc100mini, mCherry and CYC1terminator. We improved BBa_K1024002 by adding ADH1 terminator and CYC1terminator. The LuxR gene is constitutively expressed. On the other hand, activation of pLux occurs only when sufficient amounts of AHL exist in the environment. When pLux was activated, mCherry is expressed and lets us know the existence of AHL.
+We created this part with reference to BBa_K1024002in order to signal when Pseudomonas aeruginosa exists. The part is composed of pTEF, Flag, VP16, NLS, LasR, ADH1 terminator, pLasR, Cyc100mini, mCherry and CYC1terminator. We improved <bbpart>BBa_K1024002</bbpart> by adding ADH1 terminator(<bbpart>BBa_J63002</bbpart>) and CYC1terminator(<bbpart>BBa_K1462070</bbpart>). The LuxR gene is constitutively expressed. On the other hand, activation of pLux occurs only when sufficient amounts of AHL exist in the environment. When pLux was activated, mCherry is expressed and lets us know the existence of AHL.
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