Difference between revisions of "Part:BBa K3804009"
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===real sequence of the part=== | ===real sequence of the part=== | ||
As we explained in design page, our part is based on <bbpart>BBa_K1024002</bbpart>. thus, we show the sequence below.<br /> | As we explained in design page, our part is based on <bbpart>BBa_K1024002</bbpart>. thus, we show the sequence below.<br /> | ||
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Revision as of 08:08, 29 September 2021
Reporter for quorum sensing systems in yeast with qscR
We created this part with reference to BBa_K1024002 in order to signal when Pseudomonas aeruginosa exists. The part is composed of pTEF, Flag, VP16, NLS, QscR, ADH1 terminator, pQsc, Cyc100mini, mCherry and CYC1terminator. We improved BBa_K1024002 by adding ADH1 terminator and CYC1terminator and changed LuxR to QscR, pLux to pQsc. QscR is homolog of LusR. The QscR gene is constitutively expressed. On the other hands, activation of pQsc occurs only when sufficient amount of AHL exist in the environment. When pQsc was activated, mCherry is expressed and lets us know the existence of AHL.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1673
Illegal PstI site found at 2036 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1673
Illegal PstI site found at 2036 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1673
Illegal XhoI site found at 1595
Illegal XhoI site found at 1646 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1673
Illegal PstI site found at 2036 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1673
Illegal PstI site found at 2036 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1811
real sequence of the part
As we explained in design page, our part is based on BBa_K1024002. thus, we show the sequence below.