Difference between revisions of "Part:BBa K3788019:Design"

 
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===Design Notes===
 
===Design Notes===
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XbaI restriction site was delated
 
  
  

Revision as of 19:59, 28 September 2021


Expression of GFP and of the timer device under the control of the LexA-controlled promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1528
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1848
    Illegal BamHI site found at 1521
    Illegal XhoI site found at 1397
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1597
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 867
    Illegal SapI.rc site found at 1351


Design Notes

Source

colA sequence was given by LISM laboratory from CNRS. RFP coding sequence was taken corresponding to Part:BBa_E1010


References