Difference between revisions of "Part:BBa K3866037"
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===Functional Parameters===
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===Source===
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<partinfo>BBa_K3866037 parameters</partinfo>
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Synthesized by IDT.
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Revision as of 15:54, 27 September 2021


pAndersonJ23115:RBS-N20bglx-terminator

N20-bglX BBa_K3866032 under control of a constitutive promoter BBa_K3505012.

Fig.1: N20-bglX under control of a constitutive promoter.


Usage and Biology

Τhis part consists of AndersonJ23115 promoter BBa_K3505012, N20-bglX BBa_K3866032 and Double Terminator BBa_K3505017. By this way, as AndersonJ23115 is a strong constitutive promoter, bglx with the N20- signal peptide is constitutively expressed. The product protein is expected to be extracellular.

Design Notes

Τhe coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in alpha1R vector BBa_K3505008and has overhangs compatible for GoldenBraid cloning.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1700
    Illegal AgeI site found at 1922
    Illegal AgeI site found at 2111
  • 1000
    COMPATIBLE WITH RFC[1000]


Source

Synthesized by IDT.