Difference between revisions of "Part:BBa K3866033"
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Τhe coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in alpha1R vector <bbpart>BBa_K3505008</bbpart>and has overhangs compatible for GoldenBraid cloning.  
 
Τhe coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in alpha1R vector <bbpart>BBa_K3505008</bbpart>and has overhangs compatible for GoldenBraid cloning.  
  
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===Sequence and Features====
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3866033 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3866033 SequenceAndFeatures</partinfo>
  

Revision as of 14:38, 27 September 2021


pAndersonJ23115:RBS-bglX-terminator

Bglx BBa_K523002 under control of a constitutive promoter BBa_K3505012.

Usage and Biology

Τhis part consists of AndersonJ23115 promoter BBa_K3505012, bglx BBa_K523002 and Double Terminator BBa_K3505017. By this way, as AndersonJ23115 is a strong constitutive promoter, bglx is constitutively expressed. The product protein is expected to be in the periplasm.

Design Notes

Τhe coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in alpha1R vector BBa_K3505008and has overhangs compatible for GoldenBraid cloning.

Sequence and Features=


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 62
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1721
    Illegal AgeI site found at 1943
    Illegal AgeI site found at 2132
  • 1000
    COMPATIBLE WITH RFC[1000]


Source

Synthesized by IDT.