Difference between revisions of "Part:BBa K3866003"

Revision as of 19:17, 26 September 2021

Partial T5 Promoter - CuO- RBS GB compatible with A1-B2

Partial T5 promoter is a strong constitutive Promoter that can be used in the toxin-antitoxin system with the Cym repressor that our team uses in their system.

Fig.2:Partial T5 Promoter with Lactose Operator and Cumate Operator with compatible ends for Golden Braid Cloning

Usage and Biology

T5 promoter has been used for many years for protein expression in E.coli. Partial T5 promoter is used with the downstream Operator CuO.

Design Notes

The sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 BBa_K3505007 and has overhangs compatible for GoldenBraid cloning. This has position A1-B2.

Fig.1:The overhangs of this part in the GoldenBraid Grammar

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Source

Synthesized by Twist Biosciences.

References

Choi, Y. J., Morel, L., Le François, T., Bourque, D., Bourget, L., Groleau, D., Massie, B., & Míguez, C. B. (2010). Novel, versatile, and tightly regulated expression system for Escherichia coli strains. Applied and environmental microbiology, 76(15), 5058–5066. https://doi.org/10.1128/AEM.00413-10

@@ Line 5: / Line 5: @@
 Partial T5 promoter is a strong constitutive Promoter that can be used in the toxin-antitoxin system with the Cym repressor that our team uses in their system.
-[[File:T--Thessaly--Partial T5 CuO.png|700px|thumb|none|<i><b>Fig.2:</b>J23115 with RBS</i>]]
+[[File:T--Thessaly--Partial T5 CuO.png|700px|thumb|none|<i><b>Fig.2:</b>Partial T5 Promoter with Lactose Operator and Cumate Operator with compatible ends for Golden Braid Cloning</i>]]
 ===Usage and Biology===