Difference between revisions of "Part:BBa K3832007:Design"
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===References=== | ===References=== | ||
| + | [1]Timothy S. Gardner, Charles R. Cantor, James J. Collins, Construction of a genetic toggle switch in Escherichia coli Supplementary Information, Nature volume 403, pages 339–342 (2000) | ||
Latest revision as of 15:51, 26 September 2021
Toggle-switch circuit with GFP and RFP
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 2854
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 2854
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 2854
Illegal AgeI site found at 1891
Illegal AgeI site found at 3436
Illegal AgeI site found at 3548 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
sfGFP and mRFP1 are functioned as reporter here. Target gene should be inserted between CDS of reporter gene and the terminator. The efficiency of RBS followed by repressor genes are crucial to realize the bistable statement of toggle switch circuit. Trying to insert gene or other sequence between promoter and RBS is Not Recommanded.
Source
Sequences are derived from existing parts in iGEM registry. DNA samples are De-novo synthesized.
References
[1]Timothy S. Gardner, Charles R. Cantor, James J. Collins, Construction of a genetic toggle switch in Escherichia coli Supplementary Information, Nature volume 403, pages 339–342 (2000)