Difference between revisions of "Part:BBa K3715004"
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<partinfo>BBa_K3715004 short</partinfo> | <partinfo>BBa_K3715004 short</partinfo> | ||
− | PET hydrolase (PETase), which hydrolyzes polyethylene terephthalate (PET) into soluble building blocks, provides an attractive avenue for the bioconversion of plastics. Here we present the structures of a novel PETase from the PET-consuming microbeIdeonella sakaiensis. Well, | + | PET hydrolase (PETase), which hydrolyzes polyethylene terephthalate (PET) into soluble building blocks, provides an attractive avenue for the bioconversion of plastics. Here we present the structures of a novel PETase from the PET-consuming microbeIdeonella sakaiensis. Well, Super4 is a complicated mutation of PETase, which contains 11 mutation sites:S214H-I168R-W159H-S188Q-R280A-A180I-G165A-Q119Y-L117F-T140D-A80V. |
Revision as of 08:11, 26 September 2021
Super4
PET hydrolase (PETase), which hydrolyzes polyethylene terephthalate (PET) into soluble building blocks, provides an attractive avenue for the bioconversion of plastics. Here we present the structures of a novel PETase from the PET-consuming microbeIdeonella sakaiensis. Well, Super4 is a complicated mutation of PETase, which contains 11 mutation sites:S214H-I168R-W159H-S188Q-R280A-A180I-G165A-Q119Y-L117F-T140D-A80V.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 783
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 783
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 783
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 783
Illegal NgoMIV site found at 58
Illegal NgoMIV site found at 112
Illegal NgoMIV site found at 139 - 1000COMPATIBLE WITH RFC[1000]