Difference between revisions of "Part:BBa K3715105"

 
 
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__NOTOC__
 
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PET hydrolase (PETase), which hydrolyzes polyethylene terephthalate (PET) into soluble building blocks, provides an attractive avenue for the bioconversion of plastics. Here we present the structures of a novel PETase from the PET-consuming microbeIdeonella sakaiensis. Well, Super is a complicated mutation of PETase, which contains 11 mutation sites&#65306;S214H-I168R-W159H-S188Q-R280A-A180I-G165A-Q119Y-L117F-T140D-I208F.
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PET hydrolase (PETase), which hydrolyzes polyethylene terephthalate (PET) into soluble building blocks, provides an attractive avenue for the bioconversion of plastics. Here we present the structures of a novel PETase from the PET-consuming microbeIdeonella sakaiensis.  
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Well,PETase_MT1 is a complicated mutation of PETase, which contains 12 mutation sites:A40P-S54W-S92P-T113P-Q119D-Q127L-Q182I-S193R-S214F-K252L-T266P-N275F.
  
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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===Functional Parameters===
 
===Functional Parameters===
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Latest revision as of 08:09, 26 September 2021


PETase_MT1

PET hydrolase (PETase), which hydrolyzes polyethylene terephthalate (PET) into soluble building blocks, provides an attractive avenue for the bioconversion of plastics. Here we present the structures of a novel PETase from the PET-consuming microbeIdeonella sakaiensis. Well,PETase_MT1 is a complicated mutation of PETase, which contains 12 mutation sites:A40P-S54W-S92P-T113P-Q119D-Q127L-Q182I-S193R-S214F-K252L-T266P-N275F.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 874
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]