Difference between revisions of "Part:BBa K3832001"

 
Line 5: Line 5:
 
This Part is the Induced expression circuit of CDS aroG (Mutant s211f) (BBa_K3832000).It contains lacUV5 promoter and a whole expression unit of lacI protein, thus aroG-s211f can be induced by IPTG.
 
This Part is the Induced expression circuit of CDS aroG (Mutant s211f) (BBa_K3832000).It contains lacUV5 promoter and a whole expression unit of lacI protein, thus aroG-s211f can be induced by IPTG.
  
−
Our team use GoldenGate assembly to assemble the subparts into this circuit. This parts contains the BioBrick prefix and at both ends.
+
Our team use GoldenGate assembly to assemble the subparts into this circuit.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 06:49, 26 September 2021


Induced expression circuit of aroG (Mutant s211f)

This Part is the Induced expression circuit of CDS aroG (Mutant s211f) (BBa_K3832000).It contains lacUV5 promoter and a whole expression unit of lacI protein, thus aroG-s211f can be induced by IPTG.

Our team use GoldenGate assembly to assemble the subparts into this circuit.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2554
    Illegal NheI site found at 2577
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2541
    Illegal XhoI site found at 1146
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 453