Difference between revisions of "Part:BBa K3866031"
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[[File:T--Thessaly--grand2gel.png|800px|thumb|none|<i><b>Figure 3.</b>: (C=Cut, U=Uncut) Restriction digestion of α1-sbm-ygfG-ygfH with: BamHI, Expected bands: 5727bp, 2612bp, 1903bp, 1076bp, 264bp, Positive result: C1-C2-C3-C4-C5</i>]] | [[File:T--Thessaly--grand2gel.png|800px|thumb|none|<i><b>Figure 3.</b>: (C=Cut, U=Uncut) Restriction digestion of α1-sbm-ygfG-ygfH with: BamHI, Expected bands: 5727bp, 2612bp, 1903bp, 1076bp, 264bp, Positive result: C1-C2-C3-C4-C5</i>]] | ||
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===Sequence and Features=== | ===Sequence and Features=== |
Revision as of 23:49, 25 September 2021
Propionate Production Construct
Usage and Biology
This composite part consists of the following two Composite Parts:
sbm:stuffer: BBa_K3866029 & ygfG:ygfH: BBa_K3866030
Design Notes
The coding sequences were domesticated. We removed BsmBI and BsaI sites in order to be compatible with GoldenBraid and MoClo. The sequences are cloned in seva ω2 vector and have overhangs compatible for GoldenBraid cloning.
Verification of Cloning
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 4655
Illegal BamHI site found at 1148
Illegal BamHI site found at 1424
Illegal BamHI site found at 3343 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 983
Illegal AgeI site found at 3178 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 965
Illegal SapI site found at 3160
Illegal SapI.rc site found at 1883