Difference between revisions of "Part:BBa K3866026"
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The coding sequence was domesticated . We removed BsmBI and BsaI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in seva a2 vector and has overhangs compatible for GoldenBraid cloning. | The coding sequence was domesticated . We removed BsmBI and BsaI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in seva a2 vector and has overhangs compatible for GoldenBraid cloning. | ||
− | [[Image:T--Thessaly--arac- | + | [[Image:T--Thessaly--arac-sbm-term.png|900px|thumb|none|<I><b>Figure 1.</b> The first level α module of the Propionate Production: α1:ParaBAD:RBS-smb-Double terminator </i>]] |
===Verification of Cloning=== | ===Verification of Cloning=== |
Revision as of 22:07, 25 September 2021
ParaBAD:sbm:Terminator
Usage and Biology
This TU includes the AckA gene placed under the control of the arabinosed-induced promoter. The inducible promoter is used because cloning wasn't done. After the constitutive expression, the metabolism went out of balance so no colonies survied to be chosen for screening.
Design Notes
The coding sequence was domesticated . We removed BsmBI and BsaI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in seva a2 vector and has overhangs compatible for GoldenBraid cloning.
Verification of Cloning
Experimental Use and Experience
This part showed functionality at this part BBa_K3866009
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2948
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2948
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2948
Illegal BamHI site found at 1148
Illegal BamHI site found at 2236 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2948
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2948
Illegal NgoMIV site found at 3263
Illegal AgeI site found at 983
Illegal AgeI site found at 1400 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 965