Difference between revisions of "Part:BBa K3866023"
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===Source=== | ===Source=== | ||
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===Sequence and Features=== | ===Sequence and Features=== |
Revision as of 21:38, 25 September 2021
sbm - Methylmalonyl-CoA mutase GB compatible with B2-B5
Usage and Biology
Catalyzes the interconversion of succinyl-CoA and methylmalonyl-CoA. Could be part of a pathway that converts succinate to propionate.
Design Notes
The sequence was domesticated. We removed BsmBI, BsaI, BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 BBa_K3505007 and has overhangs compatible for Golden Braid cloning. This has position B2-B5.
Verification of cloning
Source
Synthesized by Twist Biosciences.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1697
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1697
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1697
Illegal BamHI site found at 985 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1697
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1697
Illegal NgoMIV site found at 2012
Illegal AgeI site found at 149 - 1000COMPATIBLE WITH RFC[1000]
References
Haller T, Buckel T, Rétey J, Gerlt JA (2000). Discovering newenzymes and metabolic pathways: conversion of succinate to propionate by Escherichia coli. Biochemistry, 39:4622–4629. https://doi.org/10.1021/bi992888d