Difference between revisions of "Part:BBa K3866019"

 
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<partinfo>BBa_K3866019 short</partinfo>
 
<partinfo>BBa_K3866019 short</partinfo>
  
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[[File:T--Thessaly--AndersonRBS-snap.png|700px|thumb|none|<i><b>Fig.2:</b>J23115 with RBS</i>]]
  
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===Usage and Biology===
 
===Usage and Biology===
  
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This translational unit is consisted of and alpha 2 vector with a stuffer. Stuffer is a part of DNA that is coding for nothing. It is used in order to swap inserts from alpha 2 or alpha2R vectors into omega vectors. This serves the GB cloning procedure
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<span class='h3bb'>Sequence and Features</span>
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===Design Notes===
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The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in alpha 2 vector <bbpart>BBa_K3505009</bbpart> and has overhangs compatible for GoldenBraid cloning.
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===Sequence and Features===
 
<partinfo>BBa_K3866019 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3866019 SequenceAndFeatures</partinfo>
  
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===Source===
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Synthesized by Twist Biosciences.
  
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===References===
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===Functional Parameters===
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<partinfo>BBa_K3866019 parameters</partinfo>
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Revision as of 17:53, 25 September 2021


Stuffer PDGB A2

Fig.2:J23115 with RBS

Usage and Biology

This translational unit is consisted of and alpha 2 vector with a stuffer. Stuffer is a part of DNA that is coding for nothing. It is used in order to swap inserts from alpha 2 or alpha2R vectors into omega vectors. This serves the GB cloning procedure



Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in alpha 2 vector BBa_K3505009 and has overhangs compatible for GoldenBraid cloning.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Source

Synthesized by Twist Biosciences.

References

i