Difference between revisions of "Part:BBa K3866020"

Revision as of 17:39, 25 September 2021

J23115-CymR-Stuffer a2

Fig.2:J23115 with RBS

Usage and Biology

This composite part is consisted of the translational unit of the strong constitutive promoter J23115 BBa_K3505012 and the Cym Repressor BBa and the transcriptional unit with the stuffer BBa_K3866019, which is a part of DNA non-coding with a length close to 150 basepairs. The stuffer is used so that we can swap the insert from the alpha 2 vector into the omega 2 vector BBa_K3505011.

Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in omega2 vector BBa_K3505008 and has overhangs compatible for GoldenBraid cloning.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 11
Illegal NheI site found at 34
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 418
Illegal XhoI site found at 199
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 101

Source

Synthesized by Twist Biosciences.

References

i

@@ Line 7: / Line 7: @@
 ===Usage and Biology===
- This composite part is consisted of the translational unit of the strong constitutive promoter J23115 <bbpart>BBa_K3505012</bbpart> and the Cym Repressor <bbpart>BBa K415202</bbpart> and the transcriptional unit with the stuffer <bbpart>BBa_K3866019</bbpart>, which is a part of DNA non-coding with a length close to 150 basepairs. The stuffer is used so that we can swap the insert from the alpha 2 vector into the omega 2 vector <bbpart>BBa_K3505011</bbpart>.
+<p>This composite part is consisted of the translational unit of the strong constitutive promoter J23115 <bbpart>BBa_K3505012</bbpart> and the Cym Repressor <bbpart>BBa K415202</bbpart> and the transcriptional unit with the stuffer <bbpart>BBa_K3866019</bbpart>, which is a part of DNA non-coding with a length close to 150 basepairs. The stuffer is used so that we can swap the insert from the alpha 2 vector into the omega 2 vector <bbpart>BBa_K3505011</bbpart>.
 ===Design Notes===
 The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in omega2 vector <bbpart>BBa_K3505008</bbpart> and has overhangs compatible for GoldenBraid cloning.