Difference between revisions of "Part:BBa K3866017"

Revision as of 17:29, 25 September 2021

PArtial T5 - CuO - MazF - double terminator

Fig.2:J23115 with RBS

Usage and Biology

This part includes the strong Partial T5 Promoter with the downstream operator Cuo. Also, it includes the toxin MazF that will be utilized to the toxin-antitoxin system and the double terminator.

Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in alpha2 vector BBa_K3505009 and has overhangs compatible for GoldenBraid cloning.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Source

Synthesized by Twist Biosciences.

References

i

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3866017 short</partinfo>
-I
+[[File:T--Thessaly--AndersonRBS-snap.png|700px|thumb|none|<i><b>Fig.2:</b>J23115 with RBS</i>]]
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
-<!-- -->
+This part includes the strong Partial T5 Promoter with the downstream operator Cuo. Also, it includes the toxin MazF that will be utilized to the toxin-antitoxin system and the double terminator.
-<span class='h3bb'>Sequence and Features</span>
+===Design Notes===
+The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in alpha2 vector  <bbpart>BBa_K3505009</bbpart> and has overhangs compatible for GoldenBraid cloning.
+===Sequence and Features===
 <partinfo>BBa_K3866017 SequenceAndFeatures</partinfo>
+===Source===
+Synthesized by Twist Biosciences.
-<!-- Uncomment this to enable Functional Parameter display
+===References===
-===Functional Parameters===
+i
-<partinfo>BBa_K3866017 parameters</partinfo>
-<!-- -->