Difference between revisions of "Part:BBa K3866003"

 
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<partinfo>BBa_K3866003 short</partinfo>
 
<partinfo>BBa_K3866003 short</partinfo>
  
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<p>Partial T5 promoter is a strong constitutive Promoter that can be used in the toxin-antitoxin system with the Cym repressor that our team uses in their system.
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[[File:T--Thessaly--AndersonRBS-snap.png|700px|thumb|none|<i><b>Fig.2:</b>J23115 with RBS</i>]]
  
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===Usage and Biology===
 
===Usage and Biology===
  
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<span class='h3bb'>Sequence and Features</span>
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===Design Notes===
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The sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 <bbpart>BBa_K3505007</bbpart> and has overhangs compatible for GoldenBraid cloning.
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This has position A1-B2.
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[[File:T--Thessaly--GB-GGAG-AATG.jpeg|700px|thumb|none|<i><b>Fig.1:</b>The overhangs of this part in the GoldenBraid Grammar</i>]]
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===Experimental Use and Experience===
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This part is used in <bbpart>BBa_K3505033</bbpart> <bbpart>BBa_K3505035</bbpart>
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===Sequence and Features===
 
<partinfo>BBa_K3866003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3866003 SequenceAndFeatures</partinfo>
  
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===Source===
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Synthesized by Twist Biosciences.
  
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===References===
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===Functional Parameters===
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<partinfo>BBa_K3866003 parameters</partinfo>
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Revision as of 15:55, 25 September 2021


Partial T5 Promoter - CuO- RBS GB compatible with A1-B2

Partial T5 promoter is a strong constitutive Promoter that can be used in the toxin-antitoxin system with the Cym repressor that our team uses in their system.

Fig.2:J23115 with RBS

Usage and Biology

Design Notes

The sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 BBa_K3505007 and has overhangs compatible for GoldenBraid cloning. This has position A1-B2.

Fig.1:The overhangs of this part in the GoldenBraid Grammar


Experimental Use and Experience

This part is used in BBa_K3505033 BBa_K3505035

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Source

Synthesized by Twist Biosciences.

===References===