Difference between revisions of "Part:BBa K3715003"

Revision as of 14:39, 25 September 2021

Super3

PET hydrolase (PETase), which hydrolyzes polyethylene terephthalate (PET) into soluble building blocks, provides an attractive avenue for the bioconversion of plastics. Here we present the structures of a novel PETase from the PET-consuming microbeIdeonella sakaiensis. Well, Super3 is a complicated mutation of PETase, which contains 11 mutation sites：S214H-I168R-W159H-S188Q-R280A-A180I-G165A-Q119Y-L117F-T140D-T266P.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 783
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 783
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 783
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 783
Illegal NgoMIV site found at 58
Illegal NgoMIV site found at 112
Illegal NgoMIV site found at 139
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3715003 short</partinfo>
-PET hydrolase (PETase), which hydrolyzes polyethylene terephthalate (PET) into soluble building blocks, provides an attractive avenue for the bioconversion of plastics. Here we present the structures of a novel PETase from the PET-consuming microbeIdeonella sakaiensis. Well, Super3 is a complicated mutation of PETase, which contains 11 mutation sites&#65306;S214H-I168R-W159H-S188Q-R280A-A180I-G165A-Q119Y-L117F-T140D-T267P.
+PET hydrolase (PETase), which hydrolyzes polyethylene terephthalate (PET) into soluble building blocks, provides an attractive avenue for the bioconversion of plastics. Here we present the structures of a novel PETase from the PET-consuming microbeIdeonella sakaiensis. Well, Super3 is a complicated mutation of PETase, which contains 11 mutation sites&#65306;S214H-I168R-W159H-S188Q-R280A-A180I-G165A-Q119Y-L117F-T140D-T266P.
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