Difference between revisions of "Part:BBa K3796206:Design"
Yang Feiyue (Talk | contribs) (→Source) |
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The promoter PgsiB comes from Bacillus subtilis subsp. subtilis str. 168. | The promoter PgsiB comes from Bacillus subtilis subsp. subtilis str. 168. | ||
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The rbs sequence information is from BBa_K2963006 and the sequence itself is synthetic. | The rbs sequence information is from BBa_K2963006 and the sequence itself is synthetic. | ||
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The bjGFP is originally chemical syhthesized and we amplified this sequence from the plasmid pRJPaph-bjGFP. | The bjGFP is originally chemical syhthesized and we amplified this sequence from the plasmid pRJPaph-bjGFP. | ||
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The rrnB terminators sequence information is from BBa_K3568009 and the sequences for rrnB T1 and T2 are carried by the plasmid pXMJ19. | The rrnB terminators sequence information is from BBa_K3568009 and the sequences for rrnB T1 and T2 are carried by the plasmid pXMJ19. | ||
===References=== | ===References=== | ||
Revision as of 09:51, 24 September 2021
PgsiB-rbs-gfp-terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We added a short sequence CCTGCAGG before bjGFP to ensure proper distance between rbs and bjGFP, leading to higher translation efficiency.
Source
The promoter PgsiB comes from Bacillus subtilis subsp. subtilis str. 168.
The rbs sequence information is from BBa_K2963006 and the sequence itself is synthetic.
The bjGFP is originally chemical syhthesized and we amplified this sequence from the plasmid pRJPaph-bjGFP.
The rrnB terminators sequence information is from BBa_K3568009 and the sequences for rrnB T1 and T2 are carried by the plasmid pXMJ19.