Difference between revisions of "Part:BBa K3796203"
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This coding region ndoA(also mazF, ydcE) encodes endoribonuclease EndoA, a toxic component of a type II toxin-antitoxin (TA) system. It encodes endoribonuclease toxin in Bacillus subtilis that is a UACAU‐specific mRNA interferase, cleavaging after the first U. | This coding region ndoA(also mazF, ydcE) encodes endoribonuclease EndoA, a toxic component of a type II toxin-antitoxin (TA) system. It encodes endoribonuclease toxin in Bacillus subtilis that is a UACAU‐specific mRNA interferase, cleavaging after the first U. | ||
Previous research has proved that its overexpression is toxic for cell growth and it functions as normal in Escherichia coli. | Previous research has proved that its overexpression is toxic for cell growth and it functions as normal in Escherichia coli. | ||
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<partinfo>BBa_K3796203 parameters</partinfo> | <partinfo>BBa_K3796203 parameters</partinfo> | ||
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+ | ===Characterization=== | ||
+ | This year, our team designed a kill switch in Corynebacterium glutamicum using ndoA from Bacillus subtilis subsp. subtilis str. 168. We aimed to test if the overexpression of ndoA in Corynebacterium glutamicum can kill the enginnered bacteria effectively, and documented our works and failures in iGEM Parts to inspire future iGEM teams for kill switch design. |
Revision as of 07:49, 24 September 2021
ndoA (endoribonuclease toxin in Bacillus subtilis)
This coding region ndoA(also mazF, ydcE) encodes endoribonuclease EndoA, a toxic component of a type II toxin-antitoxin (TA) system. It encodes endoribonuclease toxin in Bacillus subtilis that is a UACAU‐specific mRNA interferase, cleavaging after the first U. Previous research has proved that its overexpression is toxic for cell growth and it functions as normal in Escherichia coli.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Characterization
This year, our team designed a kill switch in Corynebacterium glutamicum using ndoA from Bacillus subtilis subsp. subtilis str. 168. We aimed to test if the overexpression of ndoA in Corynebacterium glutamicum can kill the enginnered bacteria effectively, and documented our works and failures in iGEM Parts to inspire future iGEM teams for kill switch design.