Difference between revisions of "Part:BBa K3796203:Design"

 
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===References===
 
===References===
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[1] Park J H ,  Yamaguchi Y ,  Inouye M . Bacillus subtilis MazF-bs (EndoA) is a UACAU-specific mRNA interferase[J]. Febs Letters, 2011, 585(15).
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[2]Simanshu, DK, Yamaguchi, et al. Structural Basis of mRNA Recognition and Cleavage by Toxin MazF and Its Regulation by Antitoxin MazE in Bacillus subtilis[J]. MOL CELL, 2013, 2013,52(3)(-):447-458.
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[3] Pellegrini O ,  Mathy N ,  Gogos A , et al. The Bacillus subtilis ydcDE operon encodes an endoribonuclease of the MazF/PemK family and its inhibitor[J]. Molecular Microbiology, 2005, 56(5).
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 +
[4] Ishida Y ,  Park J H ,  Mao L , et al. Replacement of All Arginine Residues with Canavanine in MazF-bs mRNA Interferase Changes Its Specificity[J]. The Journal of Biological Chemistry, 2013, 288(11).

Revision as of 04:37, 24 September 2021


ndoA (endoribonuclease toxin in Bacillus subtilis)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The initial start codon in ndoA is "TTG". You may consider changing or adding other start codons to get possible higher expression. For example, XJTU-China added another "ATG" before the initial sequence of ndoA in part: BBa_K3331005.


Source

Bacillus subtilis subsp. subtilis str. 168

References

[1] Park J H , Yamaguchi Y , Inouye M . Bacillus subtilis MazF-bs (EndoA) is a UACAU-specific mRNA interferase[J]. Febs Letters, 2011, 585(15).

[2]Simanshu, DK, Yamaguchi, et al. Structural Basis of mRNA Recognition and Cleavage by Toxin MazF and Its Regulation by Antitoxin MazE in Bacillus subtilis[J]. MOL CELL, 2013, 2013,52(3)(-):447-458.

[3] Pellegrini O , Mathy N , Gogos A , et al. The Bacillus subtilis ydcDE operon encodes an endoribonuclease of the MazF/PemK family and its inhibitor[J]. Molecular Microbiology, 2005, 56(5).

[4] Ishida Y , Park J H , Mao L , et al. Replacement of All Arginine Residues with Canavanine in MazF-bs mRNA Interferase Changes Its Specificity[J]. The Journal of Biological Chemistry, 2013, 288(11).