Difference between revisions of "Part:BBa K3853018"

Revision as of 07:11, 20 September 2021

MnP(S78P)

Manganese peroxidase (MnP) is the key enzyme in our degrading system. In order to improve its catalyzing ability, we tried rational design. And according to the computational redesign results, 6 mutants were chosen and tested, including their relative enzyme activity and the effect of temperature/pH/organic solvents on them. MnP(S78P) is the sixth promising mutant of MnP, possessing lower energy after mutation and would probably has a better stability.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3853018 short</partinfo>
-Manganese peroxidase (MnP) is the key enzyme in our degrading system. In order to improve its catalyzing ability, we tried rational design. And according to the computational redesign results, 6 mutants were chosen and tested, including their relative enzyme activity and the effect of temperature/pH/organic solvents on them. MnP(S78P) is the sixth promising mutant of MnP, possessing lower energy after mutation and would probably has a better stability, but finally showed a sharp decline in enzyme activity, even close to zero.
+Manganese peroxidase (MnP) is the key enzyme in our degrading system. In order to improve its catalyzing ability, we tried rational design. And according to the computational redesign results, 6 mutants were chosen and tested, including their relative enzyme activity and the effect of temperature/pH/organic solvents on them. MnP(S78P) is the sixth promising mutant of MnP, possessing lower energy after mutation and would probably has a better stability.
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