Difference between revisions of "Part:BBa K3853000"
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| − | Manganese Peroxidase (MnP) is a highly glycosylated lignin peroxidase with heme. It can oxidize | + | Manganese Peroxidase (MnP) is a highly glycosylated lignin peroxidase with heme. It can oxidize Mn<sup>2+</sup> to Mn<sup>3+</sup>, which would be chelated by ligands like oxalic acid. And the Mn3+-ligand chelate compound would be released outside the enzyme for further degrading lignin or other refractory chemicals.This gene comes from <i>Phanerochaete chrysosporium</i>. Using the <b>Thermo GeneArt</b> online tool geneoptimize, we optimize the codon of the protein gene sequence to improve its expression in Pichia pastoris. The Introduction of this gene helps our system effectively degrade PE. |
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Revision as of 14:09, 19 September 2021
Manganese Peroxidase (MnP)
Manganese Peroxidase (MnP) is a highly glycosylated lignin peroxidase with heme. It can oxidize Mn2+ to Mn3+, which would be chelated by ligands like oxalic acid. And the Mn3+-ligand chelate compound would be released outside the enzyme for further degrading lignin or other refractory chemicals.This gene comes from Phanerochaete chrysosporium. Using the Thermo GeneArt online tool geneoptimize, we optimize the codon of the protein gene sequence to improve its expression in Pichia pastoris. The Introduction of this gene helps our system effectively degrade PE.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]