Difference between revisions of "Part:BBa K3758200"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K3758200 short</partinfo> | <partinfo>BBa_K3758200 short</partinfo> | ||
| + | __TOC__ | ||
| − | + | <p> | |
| + | This part was created using the Phytobrick Entry Vector with GFP dropout (https://parts.igem.org/Part:BBa_K2560002) and was designed to be compatible with the Phytobrick assembly standard. All parts created this year were acquired via PCR from purified DNA samples using a CTAB based method (https://doi.org/10.1186/s42269-019-0066-1), by primer annealing, primer annealing, and extension reactions or synthesized via IDT/Twist. | ||
| + | </p> | ||
| − | < | + | <p> |
| − | + | All parts this year were produced to be used in the chloroplast of different plant species. For the characterization of these parts they were tested in chloroplast cell-free systems (ccfs) from either the same species or they were tested in ccfs from other plant species. Plastid parts offer the benefit of highly conserved regulatory sequences that can be used across species. Although characterizing chloroplast parts is a huge effort, in literature, it has been shown that plastid parts can be used across species to drive gene expression (https://doi.org/10.7554/eLife.13664.008). We believe that based on this knowledge we can create valuable parts that can be screened for activity in our system with the final goal of building a variety of different parts. This collection shall help combat unwanted recombination events in vivo that sometimes impede the successful functionality of the genetic design. | |
| + | </p> | ||
| − | |||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K3758200 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3758200 SequenceAndFeatures</partinfo> | ||
| − | + | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K3758200 parameters</partinfo> | <partinfo>BBa_K3758200 parameters</partinfo> | ||
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Revision as of 10:04, 19 September 2021
psbA 3-UTR (Nicotiana tabacum)
Contents
This part was created using the Phytobrick Entry Vector with GFP dropout (https://parts.igem.org/Part:BBa_K2560002) and was designed to be compatible with the Phytobrick assembly standard. All parts created this year were acquired via PCR from purified DNA samples using a CTAB based method (https://doi.org/10.1186/s42269-019-0066-1), by primer annealing, primer annealing, and extension reactions or synthesized via IDT/Twist.
All parts this year were produced to be used in the chloroplast of different plant species. For the characterization of these parts they were tested in chloroplast cell-free systems (ccfs) from either the same species or they were tested in ccfs from other plant species. Plastid parts offer the benefit of highly conserved regulatory sequences that can be used across species. Although characterizing chloroplast parts is a huge effort, in literature, it has been shown that plastid parts can be used across species to drive gene expression (https://doi.org/10.7554/eLife.13664.008). We believe that based on this knowledge we can create valuable parts that can be screened for activity in our system with the final goal of building a variety of different parts. This collection shall help combat unwanted recombination events in vivo that sometimes impede the successful functionality of the genetic design.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]