Difference between revisions of "Part:BBa K136046"
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EnvZ* is here a mutated version which constitutively phosphorylates OmpR.
 
EnvZ* is here a mutated version which constitutively phosphorylates OmpR.
  
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This part was successfully used in the composite part <partinfo>BBa_K136066</partinfo>
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A site EcoRI is present in the part which doesn't comply with the Biobrick standard. We will soon provide a new version of this part without the EcoRI site.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 04:16, 8 November 2008

envZ*

EnvZ is a transmembrane histidine kinase that, in response to osmotic changes, phosphorylates OmpR the natural inhibitor of flhDC promotor the master regulator of the flagella synthesis. EnvZ* is here a mutated version which constitutively phosphorylates OmpR.

This part was successfully used in the composite part BBa_K136066

A site EcoRI is present in the part which doesn't comply with the Biobrick standard. We will soon provide a new version of this part without the EcoRI site.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 316
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 316
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 316
    Illegal BglII site found at 236
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 316
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 316
  • 1000
    COMPATIBLE WITH RFC[1000]