Difference between revisions of "Part:BBa K3796202"
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+ | __NOTOC__ | ||
+ | <partinfo>BBa_K3796201 short</partinfo> | ||
+ | |||
+ | This part encodes phosphoenolpyruvate carboxylase(EC 4.1.1.31;GenBank: BAB98978.1),which catalyzes PEP to form OAA,which is important for the carbon flux from TCA cycle to the synthesis of amino acids. It is inserted into C.glutamicum ATCC 13032 genome with a change of RBS. | ||
+ | |||
+ | <!-- Add more about the biology of this part here | ||
+ | ===Usage and Biology=== | ||
+ | |||
+ | <!-- --> | ||
+ | <span class='h3bb'>Sequence and Features</span> | ||
+ | <partinfo>BBa_K3796201 SequenceAndFeatures</partinfo> | ||
+ | |||
+ | |||
+ | <!-- Uncomment this to enable Functional Parameter display | ||
+ | ===Functional Parameters=== | ||
+ | <partinfo>BBa_K3796201 parameters</partinfo> | ||
+ | <!-- --> |
Revision as of 11:51, 7 September 2021
Phosphoenolpyruvate carboxylase (ppc) from C.glutamicum ATCC 13032
This part encodes phosphoenolpyruvate carboxylase(EC 4.1.1.31;GenBank: BAB98978.1),which catalyzes PEP to form OAA,which is important for the carbon flux from TCA cycle to the synthesis of amino acids. It is inserted into C.glutamicum ATCC 13032 genome with a change of RBS.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1099
Illegal EcoRI site found at 1540
Illegal PstI site found at 1684
Illegal PstI site found at 1783
Illegal PstI site found at 2167 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1099
Illegal EcoRI site found at 1540
Illegal PstI site found at 1684
Illegal PstI site found at 1783
Illegal PstI site found at 2167 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1099
Illegal EcoRI site found at 1540
Illegal BglII site found at 1617
Illegal BglII site found at 2081
Illegal BamHI site found at 2179
Illegal BamHI site found at 2194
Illegal XhoI site found at 871
Illegal XhoI site found at 1516 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1099
Illegal EcoRI site found at 1540
Illegal PstI site found at 1684
Illegal PstI site found at 1783
Illegal PstI site found at 2167 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1099
Illegal EcoRI site found at 1540
Illegal PstI site found at 1684
Illegal PstI site found at 1783
Illegal PstI site found at 2167
Illegal NgoMIV site found at 1627 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2001
Illegal BsaI.rc site found at 767
Illegal SapI.rc site found at 259