Difference between revisions of "Part:BBa K3905000"

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<partinfo>BBa_K3905000 short</partinfo>
 
<partinfo>BBa_K3905000 short</partinfo>
  
Toehold switch that regulates the translation of Luciferase. When micro-RNA hsa517-5p is present, the miRNA binds to part BBa_3905001 (gen1 517-5p Anti-miRNA) which forms duplex. This duplex trigger causes the toehold switch to unfold by binding to the toehold sequence, exposing the RBS and start codon. In a composite, a reporter gene should be placed downstream here which will report when in the 'on' state.
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Toehold switch that regulates the translation of a protein, positively induced by a trigger RNA. When micro-RNA hsa517-5p is present, the miRNA binds to part BBa_3905001 (gen1 517-5p Anti-miRNA) which forms a duplex. This duplex trigger causes the toehold switch to unfold by binding to the trigger binding site, exposing the RBS and start codon and allowing translation to occur at a ribosome. In a composite, a reporter gene should be placed downstream here which will report when in the 'on' state.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 20:42, 5 September 2021


gen1 517-5p Toehold Switch

Toehold switch that regulates the translation of a protein, positively induced by a trigger RNA. When micro-RNA hsa517-5p is present, the miRNA binds to part BBa_3905001 (gen1 517-5p Anti-miRNA) which forms a duplex. This duplex trigger causes the toehold switch to unfold by binding to the trigger binding site, exposing the RBS and start codon and allowing translation to occur at a ribosome. In a composite, a reporter gene should be placed downstream here which will report when in the 'on' state.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 15
    Illegal XbaI site found at 53
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 15
    Illegal XbaI site found at 53
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 15
    Illegal XbaI site found at 53
  • 1000
    COMPATIBLE WITH RFC[1000]