Difference between revisions of "Part:BBa K079020:Experience"
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It was transformed in HL1Blue bacterial cells according to the standard protocol. One colony from each plate was picked up and let grow overnight in LB medium at 37°C. O/N culture was spinned at 6000-8000 rpm for three minutes. The supernatant was harvested and the pellet resuspended. Slides were prepared for the fluorescence bacteria image acquisition. For each slide five different views were acquired. Finally, images were elaborated with the Visual Fluo Bacteria Software. Examples of fluorescence bacteria image are shown in the following figure. The fluorescence images reveal the repression due to the presence of the Lac operator compared to K079020 (open loop circuit). | It was transformed in HL1Blue bacterial cells according to the standard protocol. One colony from each plate was picked up and let grow overnight in LB medium at 37°C. O/N culture was spinned at 6000-8000 rpm for three minutes. The supernatant was harvested and the pellet resuspended. Slides were prepared for the fluorescence bacteria image acquisition. For each slide five different views were acquired. Finally, images were elaborated with the Visual Fluo Bacteria Software. Examples of fluorescence bacteria image are shown in the following figure. The fluorescence images reveal the repression due to the presence of the Lac operator compared to K079020 (open loop circuit). | ||
| − | [[Image:ClosedLooop.jpg | | + | [[Image:ClosedLooop.jpg |300x300|center |thumbnail| K079020 Fluorescence Image]] |
| − | [[Image:Table_Closed.jpg | | + | [[Image:Table_Closed.jpg |500x300|center |thumbnail| K079020 Fluorescence Image]] |
===User Reviews=== | ===User Reviews=== | ||
Revision as of 13:08, 4 November 2008
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K079020
K079020 is a closed-loop LacI Reporter where GFP expression is auto-regulated. It was transformed in HL1Blue bacterial cells according to the standard protocol. One colony from each plate was picked up and let grow overnight in LB medium at 37°C. O/N culture was spinned at 6000-8000 rpm for three minutes. The supernatant was harvested and the pellet resuspended. Slides were prepared for the fluorescence bacteria image acquisition. For each slide five different views were acquired. Finally, images were elaborated with the Visual Fluo Bacteria Software. Examples of fluorescence bacteria image are shown in the following figure. The fluorescence images reveal the repression due to the presence of the Lac operator compared to K079020 (open loop circuit).
User Reviews
UNIQ74df5dbbd6937d74-partinfo-00000000-QINU UNIQ74df5dbbd6937d74-partinfo-00000001-QINU