Difference between revisions of "Part:BBa K165028"
 
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<partinfo>BBa_K165028 short</partinfo>
 
<partinfo>BBa_K165028 short</partinfo>
  
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This is the kozak translation initiation sequence ligated to the Sin3 Repression domain.  It can be used to build synthetic transcriptional repressing factors by ligating it to any DNA-binding domain, optionally a tandem set of fluorescent proteins to track expression, and to the [[Part:BBa_K165018|SV40 NLS +ADH1 terminator]]
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Built in the Silver Lab [http://2008.igem.org/Team:BrownTwo/Implementation/syntf Biofusion]standard.
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This is the kozak sequence ligated to the Sin3 Repression domain.  It can be used to build synthetic transcriptional repressing factors by ligating it to any DNA-binding domain, optionally a tandem set of fluorescent proteins to track expression, and to the [[Part:BBa_K165018|SV40 NLS +ADH1 terminator]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 06:10, 3 November 2008

Kozak + Sin3 Repression Domain

Built in the Silver Lab [http://2008.igem.org/Team:BrownTwo/Implementation/syntf Biofusion]standard.

This is the kozak sequence ligated to the Sin3 Repression domain. It can be used to build synthetic transcriptional repressing factors by ligating it to any DNA-binding domain, optionally a tandem set of fluorescent proteins to track expression, and to the SV40 NLS +ADH1 terminator

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1775
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 946
    Illegal SapI.rc site found at 1539