Difference between revisions of "Part:BBa K165028"
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<partinfo>BBa_K165028 short</partinfo> | <partinfo>BBa_K165028 short</partinfo> | ||
| − | This is the kozak sequence ligated to the Sin3 Repression domain. It can be used to build synthetic transcriptional repressing factors by ligating it to any DNA-binding domain, optionally a tandem set of fluorescent proteins to track expression, and to the [[Part:BBa_K165018|SV40 NLS +ADH1 terminator]] | + | This is the kozak translation initiation sequence ligated to the Sin3 Repression domain. It can be used to build synthetic transcriptional repressing factors by ligating it to any DNA-binding domain, optionally a tandem set of fluorescent proteins to track expression, and to the [[Part:BBa_K165018|SV40 NLS +ADH1 terminator]] |
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Revision as of 19:11, 2 November 2008
Kozak + Sin3 Repression Domain
This is the kozak translation initiation sequence ligated to the Sin3 Repression domain. It can be used to build synthetic transcriptional repressing factors by ligating it to any DNA-binding domain, optionally a tandem set of fluorescent proteins to track expression, and to the SV40 NLS +ADH1 terminator
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1775
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 946
Illegal SapI.rc site found at 1539