Difference between revisions of "Part:BBa K4033003"
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Ant D is extracted from Photorhabdus luminescens, and is responsible for biosynthesis of anthraquinone pigments in the nematode symbiont P. luminescens TT01.
 
Ant D is extracted from Photorhabdus luminescens, and is responsible for biosynthesis of anthraquinone pigments in the nematode symbiont P. luminescens TT01.
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<b><font size="3">Biology and Usage</font></b>
 
<b><font size="3">Biology and Usage</font></b>

Revision as of 15:48, 20 August 2021


Ant D

Ant D is extracted from Photorhabdus luminescens, and is responsible for biosynthesis of anthraquinone pigments in the nematode symbiont P. luminescens TT01.


Biology and Usage

While identifying Ant D from the anthraquinone BGC of P. luminescens TT01, phylogenetic reconstruction of the sequence alignment associate more closely with the FabF homologoues than all other KS sequences acquired from the MiBIG database. Ant D could promote the successful expression of heterologous gene in active and soluble form in E.coli. Also, Ant D is responsible of anthraquinone pigments in the nematode symbiont P. luminescens TT01, and the biosynthesis of its anthraquinone core is proposed to be enzymatically congruent with biosynthesis of actinorhodin.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 44
  • 1000
    COMPATIBLE WITH RFC[1000]


Experimental approach



References

[1] : Cummings M, Peters AD, Whitehead GFS,Menon BRK, Micklefield J, Webb SJ, et al. (2019)Assembling a plug-and-play production line for combinatorial biosynthesis of aromatic polyketides in Escherichia coli. PLoS Biol 17(7): e3000347. https://doi.org/10.1371/journal.pbio.3000347