Difference between revisions of "Part:BBa K3971002"
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| + | ===Usage and Biology=== | ||
cscA allows E.coli to grow on sucrose as a sole carbon source. Knockout strains of cscA could not grow on sucrose suggesting that it is essential for sucrose metabolism. Intracellular sucrose is hydrolyzed to glucose and fructose and it its expression is derepressed at low sucrose concentrations (0.2 %). Overexpression of cscA alone has been shown to confer sucrose utilizing ability in different E.coli strains, however with low growth rates. [1] | cscA allows E.coli to grow on sucrose as a sole carbon source. Knockout strains of cscA could not grow on sucrose suggesting that it is essential for sucrose metabolism. Intracellular sucrose is hydrolyzed to glucose and fructose and it its expression is derepressed at low sucrose concentrations (0.2 %). Overexpression of cscA alone has been shown to confer sucrose utilizing ability in different E.coli strains, however with low growth rates. [1] | ||
Overexpression has also been shown to result in leakage of the invertase, which was identified in the extracellular medium [2]. However, in wild-type strains, invertase activities are exclusively intracellular [1]. | Overexpression has also been shown to result in leakage of the invertase, which was identified in the extracellular medium [2]. However, in wild-type strains, invertase activities are exclusively intracellular [1]. | ||
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| + | [[File:T--IISER-Pune-India--cscoperon.jpg|thumb|300x370px|centre|csc operon in E.coli W for sucrose metabolism. Figure adapted from: Bruschi, Michele, et al. "A transferable sucrose utilization approach for non-sucrose-utilizing Escherichia coli strains." Biotechnology advances 30.5 (2012): 1001-1010.]] | ||
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Revision as of 16:47, 9 August 2021
cscA - Sucrose-6-phosphate hydrolase (Invertase) from E.coli W.
Usage and Biology
cscA allows E.coli to grow on sucrose as a sole carbon source. Knockout strains of cscA could not grow on sucrose suggesting that it is essential for sucrose metabolism. Intracellular sucrose is hydrolyzed to glucose and fructose and it its expression is derepressed at low sucrose concentrations (0.2 %). Overexpression of cscA alone has been shown to confer sucrose utilizing ability in different E.coli strains, however with low growth rates. [1] Overexpression has also been shown to result in leakage of the invertase, which was identified in the extracellular medium [2]. However, in wild-type strains, invertase activities are exclusively intracellular [1].
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 255
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]