Difference between revisions of "Part:BBa K3971000"
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<partinfo>BBa_K3971000 short</partinfo> | <partinfo>BBa_K3971000 short</partinfo> | ||
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| + | <!-- Add more about the biology of this part here | ||
| + | ===Usage and Biology=== | ||
PcpcB is a native cyanobacterial 'superstrong' promoter (1) for the phycocyanin β-1 subunit (cpcB) gene, whose gene product is responsible for light harvest (2). | PcpcB is a native cyanobacterial 'superstrong' promoter (1) for the phycocyanin β-1 subunit (cpcB) gene, whose gene product is responsible for light harvest (2). | ||
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We used the PcpcB-m6 promoter to express the cscB (sucrose permease) transporter to enable Synechococcus elongatus UTEX 2973 to secrete sucrose into the media when placed under salt stress. | We used the PcpcB-m6 promoter to express the cscB (sucrose permease) transporter to enable Synechococcus elongatus UTEX 2973 to secrete sucrose into the media when placed under salt stress. | ||
| − | References | + | ===References=== |
1: Zhou, Jie, et al. "Discovery of a super-strong promoter enables efficient production of heterologous proteins in cyanobacteria." Scientific reports 4.1 (2014): 1-6. | 1: Zhou, Jie, et al. "Discovery of a super-strong promoter enables efficient production of heterologous proteins in cyanobacteria." Scientific reports 4.1 (2014): 1-6. | ||
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4: Seth, Jyoti R., and Pramod P. Wangikar. "Challenges and opportunities for microalgae‐mediated CO2 capture and biorefinery." Biotechnology and bioengineering 112.7 (2015): 1281-1296. | 4: Seth, Jyoti R., and Pramod P. Wangikar. "Challenges and opportunities for microalgae‐mediated CO2 capture and biorefinery." Biotechnology and bioengineering 112.7 (2015): 1281-1296. | ||
5: Mehta, Kanika, et al. "Elevated carbon dioxide levels lead to proteome-wide alterations for optimal growth of a fast-growing cyanobacterium, Synechococcus elongatus PCC 11801." Scientific reports 9.1 (2019): 1-14. | 5: Mehta, Kanika, et al. "Elevated carbon dioxide levels lead to proteome-wide alterations for optimal growth of a fast-growing cyanobacterium, Synechococcus elongatus PCC 11801." Scientific reports 9.1 (2019): 1-14. | ||
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Revision as of 13:48, 2 August 2021
Strong constitutive cyanobacterial PcpcB-7942 mutant promoter.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]