Difference between revisions of "Part:BBa K3941000:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | This part is created from only the catalytic cellulase region of Teredinibacter turnerae T8602 celAB protein by excluding any other binding site/domain residues. | + | This part is created from only the catalytic cellulase region of Teredinibacter turnerae T8602 celAB protein by excluding any other binding site/domain residues. Codon optimization has carried out to increase production in Escherichia coli bacterium. His-tag is added to 3' end of the sequence to help purification of protein product. |
Latest revision as of 11:55, 1 August 2021
CelAB
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is created from only the catalytic cellulase region of Teredinibacter turnerae T8602 celAB protein by excluding any other binding site/domain residues. Codon optimization has carried out to increase production in Escherichia coli bacterium. His-tag is added to 3' end of the sequence to help purification of protein product.
Source
This part is created based on Teredinibacter turnerae T8602 celAB (endo-1, 4-β-D glucanase) protein and this enzyme degrades wood lignocellulose in nature.