Difference between revisions of "Part:BBa K3941002:Design"
 
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===Design Notes===
 
===Design Notes===
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This part is created based on only the coding sequence of Trichoderma reesei endoglucanase II gene by excluding introns and non-coding exon sites. Signal sequence is also removed. Codon optimization was applied. A single aminoacid mutation has been studied as the Cystein aminoacid in 99 aminoacid position is changed with Valine aminoacid.
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This part is created based on only the coding sequence of Trichoderma reesei endoglucanase II gene by excluding introns and non-coding exon sites. Signal sequence is also removed. Codon optimization has carried out to increase production in Escherichia coli bacterium. His-tag is added to 3' end of the sequence to help purification of protein product. A single aminoacid mutation has been studied as the Cystein aminoacid in 99 aminoacid position is changed with Valine aminoacid.
  
  

Latest revision as of 11:53, 1 August 2021


EGII (C99V)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is created based on only the coding sequence of Trichoderma reesei endoglucanase II gene by excluding introns and non-coding exon sites. Signal sequence is also removed. Codon optimization has carried out to increase production in Escherichia coli bacterium. His-tag is added to 3' end of the sequence to help purification of protein product. A single aminoacid mutation has been studied as the Cystein aminoacid in 99 aminoacid position is changed with Valine aminoacid.


Source

The source of this pars is Trichoderma reesei endoglucanase II gene.

References