Difference between revisions of "Part:BBa T9150"
 
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Coding region for PyrF gene. PyrF is used in the synthesis of uracil in the same pathway that Ura3 is used in yeast. Can also be used as a conterselectable marker in the presence of 5-Fluoroorotic Acid (5-FOA) and the absence of uracil.  
 
Coding region for PyrF gene. PyrF is used in the synthesis of uracil in the same pathway that Ura3 is used in yeast. Can also be used as a conterselectable marker in the presence of 5-Fluoroorotic Acid (5-FOA) and the absence of uracil.  
  
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===Information contributed by City of London UK (2021)===
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===Usage and Biology===
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Part information is collated here to help future users of the BioBrick registry.
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Metadata:
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*'''Group:''' City of London UK 2021
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*'''Author:''' Lucas Ng
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*'''Summary:''' Added information collated from existing scientific studies
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----
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PyrF codes for orotidine 5'-phosphate decarboxylase, which catalyzes the decarboxylation of orotidine 5'-monophosphate (OMP) to uridine 5'-monophosphate (UMP)
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<ref>“Rhea - Annotated Reactions Database.” n.d. Rhea. Accessed July 27, 2021. https://www.rhea-db.org/rhea/11596.
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</ref>:
  
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[[File:PyrF_reaction.png|x200px|center]]
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_T9150 SequenceAndFeatures</partinfo>
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Involved in pyrimidine metabolism, PyrF is part of the pathway biosynthesizing UMP from orotate <i>de novo</i>
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<ref>
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===Functional Parameters===
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Jensen, K. F., J. N. Larsen, L. Schack, and A. Sivertsen. 1984. “Studies on the Structure and Expression of Escherichia Coli PyrC, PyrD, and PyrF Using the Cloned Genes.” European Journal of Biochemistry 140 (2): 343–52. https://doi.org/10.1111/j.1432-1033.1984.tb08107.x.
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<partinfo>BBa_T9150 parameters</partinfo>
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</ref>.
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===References===

Latest revision as of 16:31, 27 July 2021


orotidine 5


Coding region for PyrF gene. PyrF is used in the synthesis of uracil in the same pathway that Ura3 is used in yeast. Can also be used as a conterselectable marker in the presence of 5-Fluoroorotic Acid (5-FOA) and the absence of uracil.

Information contributed by City of London UK (2021)

Part information is collated here to help future users of the BioBrick registry.

Metadata:

  • Group: City of London UK 2021
  • Author: Lucas Ng
  • Summary: Added information collated from existing scientific studies

PyrF codes for orotidine 5'-phosphate decarboxylase, which catalyzes the decarboxylation of orotidine 5'-monophosphate (OMP) to uridine 5'-monophosphate (UMP) [1]:

PyrF reaction.png

Involved in pyrimidine metabolism, PyrF is part of the pathway biosynthesizing UMP from orotate de novo [2].

References

  1. ↑ “Rhea - Annotated Reactions Database.” n.d. Rhea. Accessed July 27, 2021. https://www.rhea-db.org/rhea/11596.
  2. ↑ Jensen, K. F., J. N. Larsen, L. Schack, and A. Sivertsen. 1984. “Studies on the Structure and Expression of Escherichia Coli PyrC, PyrD, and PyrF Using the Cloned Genes.” European Journal of Biochemistry 140 (2): 343–52. https://doi.org/10.1111/j.1432-1033.1984.tb08107.x.