Difference between revisions of "Part:BBa K108020"
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DNA adenine methylase of E.coli
 
DNA adenine methylase of E.coli
  
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===Information contributed by City of London UK (2021)===
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===Usage and Biology===
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Part information is collated here to help future users of the BioBrick registry.
  
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dam causes DNA methylation within the sequence GATC, preventing cleavage by the MboI restriction endonuclease [https://pubmed.ncbi.nlm.nih.gov/368070]
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K108020 SequenceAndFeatures</partinfo>
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It is likely to act in postreplication mismatch repair [https://pubmed.ncbi.nlm.nih.gov/6308634] rather than as a member of a restriction modification system, despite sharing sequence specificity with several type II restriction methylases and endonucleases as part of the N(4)/N(6)-methyltransferase family [https://pubmed.ncbi.nlm.nih.gov/25845600]
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Furthermore, it may also be involved in DNA replication [https://pubmed.ncbi.nlm.nih.gov/3891329]
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====Reaction====
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a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N6-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-homocysteine
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[https://www.rhea-db.org/rhea/15197]
  
 
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Revision as of 10:23, 26 July 2021

Dam

DNA adenine methylase of E.coli

Information contributed by City of London UK (2021)

Part information is collated here to help future users of the BioBrick registry.

dam causes DNA methylation within the sequence GATC, preventing cleavage by the MboI restriction endonuclease [1]

It is likely to act in postreplication mismatch repair [2] rather than as a member of a restriction modification system, despite sharing sequence specificity with several type II restriction methylases and endonucleases as part of the N(4)/N(6)-methyltransferase family [3]

Furthermore, it may also be involved in DNA replication [4]

Reaction

a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N6-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-homocysteine

[5]