Difference between revisions of "Part:BBa K3513006"

Revision as of 10:19, 9 December 2020

JTi1

Promoter JTi2 consists of five repeats of 5′-GGGTTTCCCC-3′as NF-κB binding site and a minimal CMV promoter. The binding sites repeats is derived from gene vascular cell adhesion molecule 1, VCAM1. The reconstructed promoter is more than twice as efficient as the original part BBa_K2976009.

Sequence and Features

Improved by NFLS 2020

Biology

NF-κB is one of the most pathways inside the human body. This part contains five repeats of 5′-GGGTTTCCCC-3′as NF-κB binding site and a minimal CMV promoter. The binding sites repeats is derived from gene vascular cell adhesion molecule 1, VCAM1. NF-κB is the down-stream of TNF signal, so in the experiments we add TNF to induce NF-κB production in cells.

Usage

This part is used as a part improvement of BBa_K2976009.

Our new part we designed here improved the efficiency more than twice than the original part. We added a Secreted Alkaline Phosphatase (SEAP) which commonly used as a reporter gene behind the promoter. The enzyme activity of SEAP reflects the efficacy of the promoter.

The origin part is: BBa_K2976009

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The standard curve:

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Sequence and Features