Difference between revisions of "Part:BBa K3606811"
| Line 6: | Line 6: | ||
<h2>Design:</h2> | <h2>Design:</h2> | ||
| − | Since we encountered difficulties in the expression of McbABCDEFG at the beginning, we decided to check whether the McbA/B/C/D/E/F/G genes can be expressed and translated separately. We successfully cloned | + | Since we encountered difficulties in the expression of McbABCDEFG at the beginning, we decided to check whether the McbA/B/C/D/E/F/G genes can be expressed and translated separately. We successfully cloned McbF into the pGEX plasmid and transcribed it into BL21. After 5 hours of induction of McbF expression with IPTG, the whole protein SDS-PAGE was used to detect whether the McbF product was produced. |
| + | <h2>Results:</h2> | ||
| + | We have successfully expressed mcbF for the fundamental validation of the antimicrobial peptide(mccb17) expression. | ||
| + | |||
| + | https://2020.igem.org/wiki/images/f/f3/T--Fudan--Poster_Mcb-gels.jpg | ||
| + | Figure1. gel map for mcbA, mcbC, mcbE and mcbF | ||
Latest revision as of 02:38, 5 December 2020
mcbF
McbF works as channel protein responsible for efflux of MccB17.
Design:
Since we encountered difficulties in the expression of McbABCDEFG at the beginning, we decided to check whether the McbA/B/C/D/E/F/G genes can be expressed and translated separately. We successfully cloned McbF into the pGEX plasmid and transcribed it into BL21. After 5 hours of induction of McbF expression with IPTG, the whole protein SDS-PAGE was used to detect whether the McbF product was produced.
Results:
We have successfully expressed mcbF for the fundamental validation of the antimicrobial peptide(mccb17) expression.
Figure1. gel map for mcbA, mcbC, mcbE and mcbF
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]