Difference between revisions of "Part:BBa K3606814"

Revision as of 02:37, 5 December 2020

mcbBCD

Usage and Biology:

This part is an antibiotic coding gene cluster with proteins for MccB17 maturation.

Design:

Since we encountered difficulties in the expression of McbABCDEFG at the beginning, we wanted to know whether one promoter can start three consecutive genes. We successfully cloned McbBCD into the pGEX plasmid together downstream one promoter and transcribed it into BL21. After 5 hours of induction of McbG expression with IPTG, the whole protein SDS-PAGE was used to detect whether the McbG product was produced.

Results:

We have successfully expressed mcbC for the fundamental validation of the antimicrobial peptide(mccb17) expression. We've constructed the plasmid for mcbB and mcbD expression, yet the results of colony PCR is false positive thus further validation is needed. Figure1. gel map for mcbA, mcbC, mcbE and mcbF

Sequence and Features