Difference between revisions of "Part:BBa K3606806"
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We have successfully expressed mcbA for the fundamental validation of the antimicrobial peptide(mccb17) expression. | We have successfully expressed mcbA for the fundamental validation of the antimicrobial peptide(mccb17) expression. | ||
Revision as of 02:26, 5 December 2020
mcbA
Usage and Biology:
McbA is the structural gene of MccB17, which is produced by the gene cluster of McbABCDEFG.
Design:
Since we encountered difficulties in the expression of McbABCDEFG at the beginning, we decided to check whether the McbA/B/C/D/E/F/G genes can be expressed and translated separately. We successfully cloned McbG into the pGEX plasmid and transcribed it into BL21. After 5 hours of induction of McbA expression with IPTG, the whole protein SDS-PAGE was used to detect whether the McbA product was produced.
Results:
We have successfully expressed mcbA for the fundamental validation of the antimicrobial peptide(mccb17) expression.
Figure2. gel map for mcbA, mcbC, mcbE and mcbF
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]