Difference between revisions of "Part:BBa K3657048"
 
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Tetrahymena thermophila trans-splicing ribozyme catalytic core for use with two separate 5' and 3' guide RNAs,
 
Tetrahymena thermophila trans-splicing ribozyme catalytic core for use with two separate 5' and 3' guide RNAs,
 
containing the target recognition EGS and 3' exon, respectively.
 
containing the target recognition EGS and 3' exon, respectively.
 +
For more information see the design section of this part.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 03:43, 28 October 2020


Double Split-Tetrahymena Ribozyme 5'3'Trans Splicing Core

Tetrahymena thermophila trans-splicing ribozyme catalytic core for use with two separate 5' and 3' guide RNAs, containing the target recognition EGS and 3' exon, respectively. For more information see the design section of this part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 253
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 155
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]