Difference between revisions of "Part:BBa K3657037"
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Pumby proteins are modular proteins. Each subunit binds a specific ribonucleotide. | Pumby proteins are modular proteins. Each subunit binds a specific ribonucleotide. | ||
Therefore the protein can be designed to bind a specific RNA sequence.</p> | Therefore the protein can be designed to bind a specific RNA sequence.</p> | ||
| − | <p>It was planned to test the ability of this linker to bring proteins together by using Pumby proteins fused to respective Split-GFP subunits. The following parts were planned to be used: <a href="https://parts.igem.org/Part:BBa_K3657034">BBa_K3657034</a>, <a href="https://parts.igem.org/Part:BBa_K3657035"> | + | <p>It was planned to test the ability of this linker to bring proteins together by using Pumby proteins fused to respective Split-GFP subunits. The following parts were planned to be used: <a href="https://parts.igem.org/Part:BBa_K3657034">BBa_K3657034</a>, <a href="https://parts.igem.org/Part:BBa_K3657035">BBa_K3657035.</a> |
</p> | </p> | ||
<p>Due to the COVID19 pandemics no experimental data about this part was obtained. More information about the planned experiment can be found here: <a href="https://2020.igem.org/Team:Heidelberg/Pumilio_PPR">iGEM Heidelberg 2020</a></p> | <p>Due to the COVID19 pandemics no experimental data about this part was obtained. More information about the planned experiment can be found here: <a href="https://2020.igem.org/Team:Heidelberg/Pumilio_PPR">iGEM Heidelberg 2020</a></p> | ||
Revision as of 03:41, 28 October 2020
RNA Linker binding Pumby AUAGAU and Pumby AUGGUU
This part is an RNA containing binding sites for Pumby protein binding AUAGAU (BBa_K3657004) and Pumby protein binding AUGGUU (BBa_K3657005).
Usage and Biology
Pumby proteins are modular proteins. Each subunit binds a specific ribonucleotide. Therefore the protein can be designed to bind a specific RNA sequence.
It was planned to test the ability of this linker to bring proteins together by using Pumby proteins fused to respective Split-GFP subunits. The following parts were planned to be used: BBa_K3657034, BBa_K3657035.
Due to the COVID19 pandemics no experimental data about this part was obtained. More information about the planned experiment can be found here: iGEM Heidelberg 2020
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]