Difference between revisions of "Part:BBa K3657041"
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<partinfo>BBa_K3657041 short</partinfo> | <partinfo>BBa_K3657041 short</partinfo> | ||
| − | + | Split trans-splicing ribozyme catalytic core. Needs a 5' and 3' guide to assemble a functional ribozyme. | |
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | Encodes the catalytic core of the Tetrahymena thermophila self-splicing group I intron, engineered for | ||
| + | trans-splicing activity, in conjunction with additional 5' and 3' guide sequences, containing an EGS | ||
| + | complementary to the target RNA sequence on the 5' guide and a 3' exon on the 3' guide. | ||
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Latest revision as of 03:35, 28 October 2020
Split-Tetrahymena TransTransSplicing Core
Split trans-splicing ribozyme catalytic core. Needs a 5' and 3' guide to assemble a functional ribozyme.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 254
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 156
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]