Difference between revisions of "Part:BBa K3425062"
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BBa_K3425062 is a β-subunit of Qβ replicase under control of inducible pCAD promoter, and is constructed from the pCadBA operator and promoter, BBa_K3425101, the RBS for Type IIS iGEM Standard Assembly, BBa_K3425033, β-subunit of Qβ replicase with cleavable degradation tag, BBa_K3425051, as well as the double terminator BBa_B0015.
 
BBa_K3425062 is a β-subunit of Qβ replicase under control of inducible pCAD promoter, and is constructed from the pCadBA operator and promoter, BBa_K3425101, the RBS for Type IIS iGEM Standard Assembly, BBa_K3425033, β-subunit of Qβ replicase with cleavable degradation tag, BBa_K3425051, as well as the double terminator BBa_B0015.
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<br>
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<b>Refereces</b>
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<br>[1]
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<br>Yao, Y., Zhang, W., Zhang, M., Jin, S., Guo, Y., Zu, Y., Ren, K., Wang, K., Chen, G., Lou, C., and Wu, Q. (2019) A Direct RNA-to-RNA Replication System for Enhanced Gene Expression in Bacteria. ACS Synth. Biol. 8, 1067–1078
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<br><i>Note, that the authors declared the following competing financial interest(s): Y. Y., W. Z., and Q. W. have filed a patent on RNA replication system and its usage.</i>
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<br>
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'><b>Sequence and Features</b></span>
 
<partinfo>BBa_K3425062 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3425062 SequenceAndFeatures</partinfo>
  

Revision as of 03:13, 28 October 2020


β-subunit of Qβ replicase under control of inducible pCAD promoter

BBa_K3425062 is a β-subunit of Qβ replicase under control of inducible pCAD promoter, and is constructed from the pCadBA operator and promoter, BBa_K3425101, the RBS for Type IIS iGEM Standard Assembly, BBa_K3425033, β-subunit of Qβ replicase with cleavable degradation tag, BBa_K3425051, as well as the double terminator BBa_B0015.


Refereces
[1]
Yao, Y., Zhang, W., Zhang, M., Jin, S., Guo, Y., Zu, Y., Ren, K., Wang, K., Chen, G., Lou, C., and Wu, Q. (2019) A Direct RNA-to-RNA Replication System for Enhanced Gene Expression in Bacteria. ACS Synth. Biol. 8, 1067–1078
Note, that the authors declared the following competing financial interest(s): Y. Y., W. Z., and Q. W. have filed a patent on RNA replication system and its usage.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1496
    Illegal XhoI site found at 1004
    Illegal XhoI site found at 1170
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]