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Revision as of 03:00, 28 October 2020


RNA binding Pumby Protein - Motif AUAGAU


This Part is an example of a RNA-binding Pumby Protein, assembled with the Pumby Domains that are specific for the four RNA bases.

Adenine:BBa_K3657000

Guanine:BBa_K3657001

Uracil:BBa_K3657002

Cytosine:BBa_K3657003

Usage and Biology

Pumby Proteins are assembled by combining multiple RNA-base specific RNA binding Domains. They originate from the Pumilio Homology Domain (Drosophila Melanogaster), which is a RNA binding protein, that is made out of 6 repeating subunits containing three alpha helices and two imperfect subunits at the N- and C-Terminus.

<img style="width:75%" src="T--Heidelberg--measurementpprs.png">

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 184
    Illegal XhoI site found at 224
    Illegal XhoI site found at 449
    Illegal XhoI site found at 634
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]