Difference between revisions of "Part:BBa K3657005"
JuliaLanger (Talk | contribs) |
|||
| Line 17: | Line 17: | ||
Pumby Proteins are assembled by combining multiple RNA-base specific RNA binding Domains. They originate from the Pumilio Homology Domain (Drosophila Melanogaster), which is a RNA binding protein, that is made out of 6 repeating subunits containing three alpha helices and two imperfect subunits at the N- and C-Terminus. | Pumby Proteins are assembled by combining multiple RNA-base specific RNA binding Domains. They originate from the Pumilio Homology Domain (Drosophila Melanogaster), which is a RNA binding protein, that is made out of 6 repeating subunits containing three alpha helices and two imperfect subunits at the N- and C-Terminus. | ||
| + | <center> | ||
| + | <img style="width:75%" src="https://2020.igem.org/wiki/images/4/49/T--Heidelberg--measurementpprs.png"> | ||
| + | </center> | ||
<!-- --> | <!-- --> | ||
Revision as of 02:59, 28 October 2020
RNA binding Pumby Protein - Motif AUGGUU
This Part is an example of an RNA-binding Pumby Protein, assembled with the Pumby Domains that are specific for the four RNA bases.
Adenine:BBa_K3657000
Guanine:BBa_K3657001
Uracil:BBa_K3657002
Cytosine:BBa_K3657003
Usage and Biology
Pumby Proteins are assembled by combining multiple RNA-base specific RNA binding Domains. They originate from the Pumilio Homology Domain (Drosophila Melanogaster), which is a RNA binding protein, that is made out of 6 repeating subunits containing three alpha helices and two imperfect subunits at the N- and C-Terminus.
<img style="width:75%" src="
">
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 184
Illegal XhoI site found at 517
Illegal XhoI site found at 628 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]