Difference between revisions of "Part:BBa K3520018"
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==Plasmid map==
 
==Plasmid map==
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[[File:T--Athens--phimarem1_gfp_reflectin_plasmid_map.png|800px|thumb|center|Figure 1: pHimarEm1 with superfolderGFP and reflectin insert]]
  
 
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Revision as of 02:57, 28 October 2020


Recombinant pHimarEm1 with Reflectin/signalpeptide/GFP TU

Plasmid map

Figure 1: pHimarEm1 with superfolderGFP and reflectin insert

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 6663
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6663
    Illegal NheI site found at 330
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 6669
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6663
    Illegal BglII site found at 652
    Illegal BglII site found at 3191
    Illegal BglII site found at 3850
    Illegal XhoI site found at 287
    Illegal XhoI site found at 3488
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 6663
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 6663
    Illegal XbaI site found at 6678
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 1316
    Illegal AgeI site found at 2883
  • 1000
    COMPATIBLE WITH RFC[1000]