Difference between revisions of "Part:BBa K3411030"

Latest revision as of 02:45, 28 October 2020

PdcuS

To analyze the amount of nitrate, Lambert_GA iGEM 2020 needed to correlate nitrate levels to a reporter protein. Thus, Lambert iGEM needed a promoter to respond to phosphorylated NarL and report downstream GFP, a well-characterized fluorescent protein. HKUST-Rice 2015 iGEM team used the PdcuS promoter, which is supported by an article in the NCBI concerning potential promoters’ sensitivity to phosphorylated NarL levels. Phosphorylated NarL then represses the PdcuS promoter, lowering GFP levels in the presence of high levels of nitrate.

Due to a change in iGEM Parts Registry Criteria, the Lambert_GA 2020 team created Part BBa_K3411030 for the PdcuS promoter since the HKUST-Rice 2015 iGEM team was not required to create the basic parts for the corresponding composite part.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3411030 short</partinfo>
-To analyze the amount of nitrate, Lambert_GA iGEM 2020 needed to correlate nitrate levels to a reporter protein. Thus, we needed a promoter to respond to phosphorylated NarL and report downstream GFP, a well-characterized fluorescent protein. HKUST-Rice 2015 iGEM team used the PdcuS promoter, which is supported by an article in the NCBI concerning potential promoters’ sensitivity to phosphorylated NarL levels. Phosphorylated NarL then represses the PdcuS promoter, lowering GFP levels in the presence of high levels of nitrate.
+To analyze the amount of nitrate, Lambert_GA iGEM 2020 needed to correlate nitrate levels to a reporter protein. Thus, Lambert iGEM needed a promoter to respond to phosphorylated NarL and report downstream GFP, a well-characterized fluorescent protein. HKUST-Rice 2015 iGEM team used the PdcuS promoter, which is supported by an article in the NCBI concerning potential promoters’ sensitivity to phosphorylated NarL levels. Phosphorylated NarL then represses the PdcuS promoter, lowering GFP levels in the presence of high levels of nitrate.
 Due to a change in iGEM Parts Registry Criteria, the Lambert_GA 2020 team created Part BBa_K3411030 for the PdcuS promoter since the HKUST-Rice 2015 iGEM team was not required to create the basic parts for the corresponding composite part.